Massage intervention on constipation rat c-kit protein expression.doc

 PAGE \* MERGEFORMAT 15 Massage intervention on constipation rat c-kit protein expression Authors: Zhao Shu, Jin-Gen Lu, Yong-Qing Cao [Abstract] Objective To study the rat hind limb activity and the relevance of constipation and massage intervention on the distal colon c-kit expression, to study the mechanism of manipulation in the treatment of constipation. Methods cut off hind femur in rats, the establishment of constipation model, by the massage treatment, the rats in each group were extracted from distal colon total cellular protein using Western blot (Western blot) protein in each group were detected by semi-quantitative c-kit protein expression in specimens changes. The results of Western blotting showed constipation rat distal colon c-kit protein expression was significantly decreased (P amp;lt;0.01); massage intervention, c-kit protein expression increased significantly (P amp;lt;0.01). Conclusion reduced activities in rat hind limb caused constipation, can rat distal colon c-kit protein expression was decreased; The massage intervention, can constipation rat distal colon c-kit protein expression significantly increased, suggesting that massage therapy constipation and cajal interstitial cells (ICC) the number of change. [Keywords:] Constipation c-kit Western blot massage Abstract: ObjectiveTo approach the relativity of constipation with the decrease of leg activity, and the effect of manipulation on the expression of c-kit protein in rat’s colon with constipation. MethodsModels of constipation were made by cutting both femur of rat. After manipulation was done , expression of c-kit protein was detected by western-blotting. ResultsThe expression of c-kit protein of rat’s colon was obviously reduced on model group (P amp;lt;0.01). The expression of c-kit protein of rat’s colon was obviously increased after treating of manipulation (P amp;lt;0.01). ConclusionThe expression of c-kit protein of rat’s colon was obviously reduced by decreasing the activi