Metabotropic glutamate receptor 5 in acute cerebral ischemia changes.docVIP

 PAGE \* MERGEFORMAT 13 Metabotropic glutamate receptor 5 in acute cerebral ischemia changes OF: ZHANG Mao-lin Liu Junping Yang Qiaolian social Wu Yuehua Zhou [Abstract] Objective: To observe the cerebral cortex in the metabotropic glutamate receptor 5 (metabotropic glutamate receptor 5, mGluR5 in the different periods of ischemia and dynamic changes of expression of the law, investigate the changes in acute cerebral ischemia significance. Methods: 35 male Wistar rats were randomly divided into normal control group, cerebral ischemia group 1h, 3h group, 6h group, 12h group, 24h group (both surgical group) and their corresponding sham control group , the use of middle cerebral artery occlusion (MCAO model, time points to the above provisions brain tissue, HE stain, with an optical microscope level neurons with immunohistochemistry to determine the strength of positive neurons, with morphology analysis system to detect the expression of the gray value of mGluR5. Results: The surgical group were higher than mGluR5 expression in sham group and control group, compared with normal control group, ischemia and 1h the expression that is increased to 12h peak level. and sham operation group and the control group had no statistical significance. Conclusion: Acute cerebral ischemia can lead to upregulation of mGluR5, suggesting that mGluR5 in the focal cerebral ischemic injury, may contribute to cerebral ischemic disease development. but with previous studies, we hypothesized that the increase may also be a for the clearance of glutamate (Glu and the response of higher organisms is to maintain a balance of self-protection mechanism. [Keywords:] cerebral ischemia, metabotropic glutamate receptor 5 Abstract Objecive: To observe the expression of mGluR5 and the dynamic regulation in the cortex of rats with focal cerebral ischemia in different phases and to investigate its significance in acute ischemia. Methods: Thirty-five male Wistar rats were randomly divided