Methods to extract DNA from blood of.doc

 PAGE \* MERGEFORMAT 10 Methods to extract DNA from blood of [Abstract] Objective: To study the DNA extracted from blood method to apply. Methods: Venous blood samples taken, respectively, anticoagulation with or without anticoagulant treatment, extracted DNA, by electrophoresis and PCR testing. Results: The coagulation and anticoagulation of DNA yield and purity, respectively (40.2 ± 8.86) mg DNA / L blood, and (1.87 ± 0.11) mg DNA / L, (39.1 ± 10.2) mg DNA / L blood, and (1.92 ± 0.12) mg DNA / L. All samples have a high molecular weight DNA, from both DNA samples can be easily amplified by the first t  PA gene intron 8 of allele 2 Alu normality, hence the integrity and reliability of the extracted DNA is a The. Conclusion: This method is fast, simple, effective, non-toxic fresh blood and clotting from the extract DNA, suitable for clinical testing and molecular biological research. [Keywords:] DNA extraction; blood; DNA testing DNA Isolation by Simple Rapid and Innocuous Method from Blood Useful in Clinical Molecular Testing Abstract: Objective To study the method of DNA isolation from blood for application. Methods DNA was isolated and determinated by agarose gel electrophoesis and PCR from antiagglutinating and agglutinating blood samples that were withdrawn from auricular veins. Results The yields and purity of DNA isolated from clotted blood and antiagglutinating blood were (40.2 ± 8.86) mg DNA / L blood and (1.87 ± 0.11), (39.1 ± 10.2) mg DNA / L blood and (1.92 ± 0.12). The DNA that we isolated from all samples has high molecular weight and by PCR the dimorphism of Alu alleles of the 8th intron of t  PA was easy to be obtained, so it was complete and reliable. Conclusion The results showed this method is rapid, easy, efficient and innocuous for isolation of DNA from clotted and fresh blood and it is suit for clinical testing and molecular biology study. Keywords:: DNA isolation; Blood; DNA determination Routine clinical tes