Mice HMGN2 interfering vector psilence cmv 4.1 HMGN2 Construction and identification of moderating effects in vivo.doc

 PAGE \* MERGEFORMAT 13 Mice HMGN2 interfering vector psilence cmv 4.1 HMGN2 Construction and identification of moderating effects in vivo Of: Han Cao Yue Qin Denglu Xia Zhao Jing Wu Guixia Huang Xiang-Ning Chen Shanze Fanbolixia [Abstract] Objective: To construct the plasmid in mice HMGN2 interference psilence cmv 4.1 HMGN2, to further clarify the biological role of HMGN2. Method: HMGN2 shRNA fragments annealed with mouse psilence cmv 4.1 link, feeding pregnant mice, tail vein injection shRNA expression plasmid into the extraction 8.5d embryo RNA, using RT PCR detection of the expression of fetal HMGN2 identified. Results: DNA sequencing showed that psilence cmv 4.1 HMGN2 interference plasmid was successfully constructed, RT PCR showed higher expression HMGN2 fetus in a wide range 8.5d expression, and Psilencer cmv 4.1 HMGN2 after intravenous injection of significant inhibitory effect. CONCLUSION: The successful construction of 8.5d fetal HMGN2 genes interfere with the efficiency of significant interference psilencer 4.1 HMGN2 plasmids for further study of gene function HMGN2 foundation. [Keywords:] HMGN2; Psilencer c mv 4.1 HMGN2 HMGN2 chromosome structure is composed of points, may maintain the configuration of DNA molecules, gene activation. The human genome there are multiple and HMGN2 the cDNA sequences with the same copy, now that as many as 35-50 copies of [ 1], that belongs to a gene encoding HMGN2 multigene family, and this multi-gene family members are not expressed in most of the fake protein genes, are processed pseudogenes (Processed Pseudogene). This multi-gene family is generally the key genes regulating cell function, often referred to as housekeeping genes (Housekeeping Gene) [2,3]. transgenic mice currently available through the methods were found in the human HMGN2 functional multi-gene family, only one gene, it One located in chromosome 1P36.1 [4]. full 3450bp, consists of six exons, the upstream is rich in GC (75%) of the regio