Mesenchymal stem cells into bone cells in proteomic analysis.doc

 PAGE \* MERGEFORMAT 6 Mesenchymal stem cells into bone cells in proteomic analysis [Keywords:] Proteomics; mesenchymal stem cells; protein expression Proteomic analysis of human bone marrowderived mesenchymal stem cells induced to differentiate into osteoblasts [Abstract] AIM: To analyze the difference of protein profiles between human bone marrowderived mesenchymal stem cells (MSCs) and osteogenic differentiated MSCs by proteomic approach. METHODS: The MSCs were isolated primarily from bone marrow of adults and detected in cell purity by flow cytometry analyzing cell phenotypes and in cell function by multilineage potential test. The undifferentiated MSCs and osteogenic differentiated MSCs over a period of 14 d were collected and cell total proteins were extracted for finding out the differential expression by proteomic analysis including 2D gel electrophoresis and Peptide Mass Fingerprinting technology. RESULTS: Twentythree differentially expressed proteins were identified by MALDITOFMS analysis. CONCLUSION: Proteomic approach is a high throughput method to screen the key proteins which play important roles in MSCs osteogenic differentiation. [Keywords] proteomics; mesenchymal stem cells; protein expression [Abstract] Objective: To study the Proteomics Analysis of human bone marrow-derived mesenchymal stem cells (MSCs) directed differentiation of osteoblast-induced changes in cell protein expression profile. Methods: from human bone marrow cells isolated from MSCs, through the cell phenotype and multi-differentiation ability to determine the purity and functional identification of MSCs, the use of directional induction of osteoblast differentiation culture system, at 14 d after differentiation were extracted from undifferentiated cells and differentiate into cells, total protein, two-dimensional electrophoresis analysis to determine the protein point of difference by digestion Across protein, peptide mass fingerprint identification of differences in pro