Mice acute myeloid - monocytic leukemia WEHI-3 cell surface expression of immune response molecules function of Fas ligand.doc

 PAGE \* MERGEFORMAT 17 Mice acute myeloid - monocytic leukemia WEHI-3 cell surface expression of immune response molecules function of Fas ligand Author: Ling-Bo Liu, Li Wei-ming, HE Wei, ZOU 【Abstract】 In order to study mice with acute myeloid - monocytic leukemia cell line WEHI-3 cell surface of immune response molecules Fas, Fas ligand (FasL), CD80 molecule expression and function of FasL, the application flow cytometry WEHI-3 cells, surface Fas, FasL, and CD80 molecule expression, while using tritium thymidine (3H-TdR) incorporation detected FasL function. The results showed that: WEHI-3 cell surface expression of CD80 and Fas, respectively (5.06 ± 0.41)%, (6.75 ± 2.31)% (n = 5), but FasL expression rate (63.73 ± 5.23)% (n = 5) When WEHI-3 (effector cells, E), and Fas + YAC-1 cells (target cells, T) in order to 3:1,10:1 and 30:1 when the mixed culture, YAC-1 cell apoptosis rates were (26 ± 4.5)%, (35 ± 3.2)% and (43 ± 2.7)% (n = 5). Conclusion: WEHI-3 cells, high expression of FasL, with low expression of Fas and CD80, and can induce Fas + YAC-1 cell apoptosis. Keywords: acute myeloid leukemia Expression of Immune Response Molecules and Function of Fas Ligand on Surface of AML WEHI-3 Cells Abstract The purpose of this study was to investigate the expression of Fas, Fas ligand (FasL) and CD80 and function of FasL on the surface of acute myelomonocytic leukemia cells from WEHI-3 line. The expression of Fas, FasL and CD80 on the surface of WEHI-3 were detected by flow cytometry, the apoptosis of YAC-1 cell induced by FasL on the surface of WEHI-3 were detected by 3H-TdR incorporation. The results showed that the expression rate of Fas, FasL and CD80 on the surface of WEHI-3 cells were (6.75 ± 2.31)% (n = 5), (63.73 ± 5.23)% (n = 5) and (5.06 ± 0.41)% (n = 5) respectively. The apoptosis rate of YAC - 1 cells (target cells) co-cultured with WEHI-3 cells (Effector cells) at the rate of 1:3,1:10 and 1:30 were (26 ± 4.5)%, (35 ± 3.2