Micron grains of quartz micro-column electrophoresis Preparation and electrophoretic separation.doc

 PAGE \* MERGEFORMAT 16 Micron grains of quartz micro-column electrophoresis Preparation and electrophoretic separation Of: Lian-He Youzhao Xie Gan fifty-two marine Gao Yong Wang Xiaokui [Abstract] studied the 2 mm diameter quartz tube filled with uniform grains of quartz-micron micro-column gel electrophoresis the feasibility of preparation and electrophoretic separation. Quartz-micron grain hydrothermal method. Containing 30% methanol 1  5 mmol / L Na2HPO4 the electrophoresis buffer (pH 11  5, no derivative of tryptophan, phenylalanine and tyrosine were separated by electrophoresis and micro-column UV absorption detection. The detection limits were 0  038, 0  21 and 0  20 μmol / L, the separation efficiency of tryptophan to 4  4 × 104 plates / m, electrophoretic micro-column sample capacity of 35 μL, and the better the separation reproducibility. for failure to fill the quartz particles , average particle size of 360 μm filled with quartz sand and quartz-micron grain size 9 μm length of the electrophoretic micro-column thermal effects are discussed. The experimental results show that in the electrophoretic micro-column filled with quartz-micron grain size in the large column diameter electrophoresis suppress the thermal effect increased sample capacity and improve detection sensitivity. The micro-column gel electrophoresis can be used as on-site, real-time and portable electric current total analysis systems and efficient separation method, suitable for large volume and low concentration samples. [Keywords:] micro-column electrophoresis, micro quartz grains, hydrothermal synthesis, thermal effects, the electric current total analysis system, amino acids Abstract The preparation of electrophoretic microcolumn and its application to the electrophoretic separation of amino acids with a 2 mm ID fused silica microcolumn packed with uniform quartz microncrystal prepared by hydrothermal synthesis are reported. With 1  5 mmol / L disodium phosphate