Microwave Mutation Breeding of tetracyclines antibiotics strains of the exploration and application of.docVIP

 PAGE \* MERGEFORMAT 7 Microwave Mutation Breeding of tetracyclines antibiotics strains of the exploration and application of Author: Chen Wang Chunkui Guo Na Li Fang Wu [Abstract] using microwave irradiation treatment tetracyclines antibiotic production strains - Health Streptomyces aureofaciens (Streptomyces aureofaciens). When the microwave power of 1200W, pulse frequency of 2450MHz, the most obvious effect of radiation mutagenesis 4s. 01  2  17 strains after treatment were 4  14 # strain. The strain characteristics of the fine, 15m3 fermentation jar test, 37 batch fermentation potency average 5.9% higher than the control strains. Dealing with the other strains of antibiotic producing strains tetracyclic 44 #, 27 # bred strains, shake-flask titers re-testing for more than 10.5% higher than the control. [Keywords:] tetracyclines antibiotics; microwave; mutagenesis; fermentation; Health Streptomyces aureofaciens Microwave in biology is mainly used for sterilization, to stimulate plant growth and seed germination [1]. As people of the biological effects of microwave and microbiological characteristics of in-depth knowledge of microwave micro-organisms in the industrial application of mutation breeding are also increasingly widespread [2], and ultraviolet rays, ion implantation, anti-cancer drugs, dyes and other methods dealing with bacteria species compared to microwave treatment method is simple, low cost, high security, less pollution to environment and so on. In this study explores the microwave on tetracyclines antibiotics production strains - Health Streptomyces aureofaciens (Streptomyces aureofaciens) the mutagenic effects. 1 Materials and methods The starting strain for the 01  2  17 and 44 # (tetracyclines antibiotics producing strains). 1.2 medium Tetracyclines antibiotics strain selection routine medium. 1.3 mutagenic treatment and screening methods (1) Preparation of spore suspension of the starting strain of tomato to grow a good