Microwave radiation - solvent extraction of total agricultural auspicious Flavonoids.doc

 PAGE \* MERGEFORMAT 8 Microwave radiation - solvent extraction of total agricultural auspicious Flavonoids Of: Huang Xiaowen, Fan light in Asia, Zhangyu Fan, Hua-Jun Fan [Abstract] Objective To optimize the total flavonoids of agricultural auspicious Microwave - solvent extraction process conditions. Method based on single-factor test to Vitexin as indicators of total flavonoid content, optimized by orthogonal test microwave power, microwave radiation time, extraction temperature, extraction time and other factors. The results of microwave radiation the best extraction solvent extraction process as follows: microwave power 560 W, microwave irradiation time 2 min, liquid to solid ratio 20:1, temperature 70 , extraction time 2.0 h. Conclusion and compared to conventional reflux extraction method, using microwave radiation medicine after solvent extraction, the extraction of total flavonoids was significantly increased; optimized process is stable and feasible. [Keywords] microwave radiation; agricultural auspicious; total flavonoids; Vitexin; orthogonal design Abstract: Objective To optimize the experimental conditions of microwave irradiated reflux extraction of total flavonoids from Crotalaria sessiliflora L. Method Orthogonal test was designed including microwave power, irradiation time, extraction temperature, extraction time based on single factor experiments with vitexin as index constituent of the total flavonoids. Results Optimum conditions were resulted with 20 times of methanol extracting for 2 h at 70 after irradiation of the medical materials for 2 min under microwave power 560 W. Conclusions Compared with the conventional solvent reflux extraction, the yield of total flavonoids from Crotalaria sessiliflora L. was improved greatly after the pretreatment of microwave irradiation of the medicinal materials, which was feasible, easy and reliable. Keywords:: microwave irradiation; Crotalaria sessiliflora L; total flavonoids; vitexin; orth