Mifepristone induced apoptosis in human cervical carcinoma Hela cell research.docVIP

 PAGE \* MERGEFORMAT 13 Mifepristone induced apoptosis in human cervical carcinoma Hela cell research [Abstract] Objective mifepristone (MIF on human cervical cancer Hela cells induced apoptosis and its mechanism. Methods Cultured human cervical cancer Hela cells, flow cytometry analysis of different concentrations of mifepristone on Hela cells withered death rate and cell cycle distribution, using Western blot, Mifepristone on Hela cells NF-B p65 protein expression. The results 5,10,20 mol / L of mifepristone all can Hela apoptosis rate was increased, the S phase, G2 / M phase cells decreased, G0/G1 phase cells increased significantly, and dose-dependent manner, with increasing concentrations of mifepristone, Hela cells, NF- B p65 protein levels decreased gradually. Conclusions Mifepristone may NF-B signaling pathway by down expression of NF-B p65 protein, which regulate the cell cycle and induce apoptosis in Hela cells. [Keywords:] human cervical carcinoma, Hela cells, mifepristone, apoptosis, cell cycle, NF-B, p65 Abstract: Objective To investigate the regulatory effect of mifepristone on the cell apoptosis of human cervical cancer cell line Hela and the underlying mechanisms. Methods Human cervical cancer cell line Hela was cultured in vitro. Flow cytometry analysis technique was used to determine the changes of cell cycle distribution and cell apoptosis percentage after treatment with different concentrations of mifepristone. Western blot was performed to examine the effect of mifepristone on the expression of NF-B p65 protein of Hela cell line. Results 5, 10 and 20 mol / L mifepristone significantly increased the cell apoptosis percentage and the proportion of cells in G0/G1 phase of Hela cells and decreased the proportion of cells in S and G2 / M phases in a dose-depending manner. With the increase of mifepristone concentrations, the expression of NF-B p65 was gradually downregulated in Hela cells. Conclusion Mifepristone can regulate cell cycle