Milk by High Performance Liquid Chromatography of nuclear Sanjie Icariin capsules.docVIP

 PAGE \* MERGEFORMAT 8 Milk by High Performance Liquid Chromatography of nuclear Sanjie Icariin capsules [Abstract] Objective To establish a milk capsules nuclear Sanjie method for determination of icariin. Methods using high performance liquid chromatography (HPLC) method, ODS C18 (4.6 mm * 250 mm, 5 μ m), with acetonitrile - water (30:70) as mobile phase, flow rate 1.0 ml / min, detection wavelength 270 nm. Results of icariin in the 10.92 ~ 65.52 μ g * ml-1 within the concentration range of the sample with peak area showed good linear relationship (r = 0.999 9), the average recovery was 99.30% (RSD = 0.45%, n = 6). Conclusions The method is simple, sensitive, accurate, and can be used for milk quality control of nuclear Sanjie capsules. [Keywords:] high-performance liquid chromatography; milk nuclear Sanjie capsule; icariin Abstract: ObjectiveTo establish the content determination method of Icariin in Ruhe Sanjie capsules by HPLC. MethodsHPLC analysis was carried out at the following conditions: Cromasil C18 column (4.6 mm * 250 mm, 5 μ m), mobile phase: Acetonitrile-Water (v / v) = 30:70, the detection wavelength at 270 nm, the flow rate at 1.0 ml * min-1. ResultsThe standard curve was linear over the range of 10.92 ~ 65.52 μ g * ml-1 (r = 0.999 9), and the average recovery was 98.30% with RSD of 0.45% (n = 6); ConclusionThe method is simple, sensitive and accurate, and available for control of Ruhe Sanjie Capsules. Keywords:: HPLC; Ruhe Sanjie Capsules; Icariin Milk nuclear Sanjie [1] capsule Shugan Jieyu, Ruanjian Sanjie, Qi and blood circulation effect. All side by angelica, astragalus, light arrowhead, Loulu, Bupleurum, turmeric, kelp, seaweed, epimedium, deer grass title composed of 10 Herbs and clinical for the treatment of breast cystic hyperplasia, breast pain, breast fiber adenoma and male breast development disorders. And the variety is not ‘Chinese Pharmacopoeia’ standard, ministerial standards, only TLC identification, there is no main