Mixed enzyme digestion test drowning experimental study of organ diatoms.doc

 PAGE \* MERGEFORMAT 17 Mixed enzyme digestion test drowning experimental study of organ diatoms [Abstract] Objective To explore the use of mixed enzyme digestion from drowning by lung, liver, kidney, spleen and other organs in the detection of diatoms, for the distinction of drowning during his lifetime or after death cadavers into the water to provide a scientific basis. Methods The production of animal experiments drowning organ samples, selected through a single enzyme digestion of several digestive ability, cheaper enzymes, will be proportional mixture of several enzymes to digest organs. At the same time comparison acid chemical digestion method. The best results of the lungs as a digestive enzyme combination collagenase Ⅰ , proteinase K, hyaluronidase and trypsin; the liver, the best combination of digestive enzymes as collagenase Ⅱ , proteinase K, hyaluronidase and trypsin; kidney of the most good combination of enzymatic digestion and proteinase K, hyaluronidase and trypsin; spleen the best combination of digestive enzymes as collagenase Ⅰ , collagenase Ⅱ , proteinase K, hyaluronidase. Conclusion mixed enzyme digestion tests drowning organs of diatoms in terms of speed, security, environmental protection, detection rates are better than acid digestion method, should be within the scope of Criminal promote the use of science and technology. [Keywords:] hybrid enzyme; digestion; diatoms; drowning An experimental study on detection of diatom in drowning organ of bodies by multi-enzymatic digestion method [Abstract] Objective The purpose of this study is to evaluate the reliability and applicability of quantitative and qualitative diatom analysis by multi-enzymatic digestion method in the diagnosis of drowning organ of bodies.Methods This study included beforehand samples, normal control samples, control samples of experiment and experimental samples. Organ samples were treated by both chemical and multi-enzymatic methods, the first one using c