MN9D dopaminergic cell injury model based.doc

 PAGE \* MERGEFORMAT 14 MN9D dopaminergic cell injury model based Study: Hong Yan Zhang Xuewen Liu Huaidong button high Dianshuai [Abstract] Objective To establish the in vitro dopamine (dopamin, DA) neuronal cell line MN9D cells can damage model. Methods Cell culture conditions in vitro, in culture medium with different concentrations of 6 - hydroxydopamine (6-hydroxydopamine ,6-OHDA) role in MN9D cells after 30 min.24 h, MTT colorimetric assay MN9D cell viability, Hoechst 33258 nuclear staining and flow cytometry apoptosis in MN9D cells. Results 6-OHDA caused a concentration dependent manner MN9D cell apoptosis, resulting in decreased cell survival .200 mol / L concentration of the role of 6-OHDA 30 min, after 24 h culture, MN9D cell viability decreased to about 68.8%, apoptosis rate increased to about 37.8%. Conclusion 6-OHDA can cause MN9D cell viability decreased and apoptosis increased, resulting in DA similar to Parkinson’s disease when the performance of neuronal injury. [Keywords:] dopaminergic neurons; 6 - hydroxydopamine; MN9D cells; Parkinson’s disease Abstract: Objective To establish the cell injury model of dopaminergic cell line MN9D in vitro. Methods MN9D cells were exposed to different concentrations of 6-hydroxydopamine (6-OHDA) for 30 min in vitro, and 24 h later, the survival of MN9D cells was detected with MTT chromatometry, and the MN9D cells apoptosis was determined with Hoechst 33258 staining and flow cytometry.Results 6-OHDA caused the apoptosis of MN9D cells in a concentration-dependent manner: the survival rate of cells decreased approximately to 68.8% while the apoptotic percentage of cells increased to 37.8% 24 h after a 30 min exposure to 200 mol / L 6-OHDA. Conclusion 6-OHDA could induce an decrease in the survival rate of MN9D cells and an increase in their apoptosis percentage, causing an injury to MN9D cells in vivo in a form similar to the injury model of dopaminergic neurons in Parkinson’s disease (PD). Keyword