Modified guanidine hydrochloride extraction of umbilical cord blood DNA for the HLA Genotyping.doc

 PAGE \* MERGEFORMAT 16 Modified guanidine hydrochloride extraction of umbilical cord blood DNA for the HLA Genotyping Author: Tang Xue-Wei, Liao, Li Yan, Xiexing Mei, Huang Yi-ning 【Abstract】 In order to compare the classic guanidine hydrochloride (Gu * HCl) extraction method and improved DNA extraction GuHCl extracted DNA method in the effect of umbilical cord blood DNA to explore these two approaches in the umbilical cord blood histocompatibility antigen (HLA) genes Typing in the application of DNA extraction method using guanidine hydrochloride Guanidine hydrochloride extraction and improved extraction of DNA samples of 72 cases of umbilical cord blood DNA, and were determined the concentration and purity of DNA, using sequence-specific primers PCR method ( PCR-SSP) compare the two methods extracted DNA. The results showed that: two kinds of DNA were successfully extracted from umbilical cord blood; quality monitoring based on the DNA, found that DNA extracted with modified guanidine hydrochloride in quality than the GuHCl method well; for HLA genotyping, the improvement of guanidine hydrochloride extraction DNA method can be raised to reduce non-specific bands appear. Conclusion: The modified DNA GuHCl extraction method is not only simple, cost reduction, but at least be able to extract the blood of high-purity DNA, to reduce non-specific bands appear. The Act fully meet the daily umbilical cord blood bank samples of DNA extraction and HLA genotyping of cord blood requirements. Keywords: DNA extraction Modified Guanidine Hydrochloride Method for DNA Extraction from Cord Blood Used in HLA Genotyping Abstract To compare two different methods for extracting genomic DNA from cord blood and to evaluate their applications for HLA genotyping, the genomic DNA from 72 samples was extracted by guanidine hydrochloride (Gu * HCl) and modified guanidine hydrochloride, the DNA yield and purity were evaluated by spectrophotometry and detec