Molecules inhibit the release of exogenous carbon monoxide inflammatory response in sepsis.doc

 PAGE \* MERGEFORMAT 29 Molecules inhibit the release of exogenous carbon monoxide inflammatory response in sepsis On: Sun Bingwei, Chen Xi, Kazuhiro Katada, Richard F Potter, Gediminas Cepinskas [Abstract] Objective: To investigate the release of exogenous carbon monoxide molecules on the inflammatory response in sepsis and possible mechanism of inhibition. Methods: cecal ligation and perforation model of sepsis in mice, the use of exogenous carbon monoxide releasing molecule (CORM 2 , 8 mg / kg body weight, tail vein injection to intervene. detection of liver, lung myeloperoxidase (MPO) activity. application of endotoxin (LPS, 10 g / ml) stimulated human umbilical vein endothelial cell inflammatory model, the use of release of exogenous carbon monoxide molecule (CORM 2,10 ~ 100 mol / L to intervene. detection of nuclear factor B (NF B activity, the expression of endothelial cell adhesion molecules, oxidation products, NO products and polymorphonuclear leukocyte adhesion to endothelial cells effect. Results: cecal ligation and perforation model of sepsis in mice using carbon monoxide releasing molecules of exogenous intervention liver and lung MPO activity decreased significantly. CORM 2 inhibited LPS stimulation resulted in increased activity of NF B. Meanwhile, NO products drop , endothelial cells inhibited the expression of ICAM 1, leukocyte adhesion on endothelial cells was significantly inhibited. Conclusion: the release of exogenous carbon monoxide molecules by inhibiting NF B activity, protein and NO inhibition of ICAM 1 expression, inhibition of leukocyte adhesion on endothelial cells and thus inhibit the inflammatory response in sepsis. [Keywords] carbon monoxide, cecal ligation and perforation, inflammation, nuclear factor B CLP (cecal ligation and puncture) may induce the activation of an inflammatory cascade, cause damage to multiple organs distant from the original burn wound and may lead to sepsis and multiple organ failure [1]. There