MPTP caused by HJD on prevention and treatment of Parkinsons disease in mice.doc

 PAGE \* MERGEFORMAT 13 MPTP caused by HJD on prevention and treatment of Parkinson’s disease in mice Study: Cold Xin Li Ruifang paragraph Zhang Yang Zhou Qiuli Kim Jin Wang Wenjia [Abstract] Objective To observe HJD (HJD of 1-methyl 4-phenyl 1,2,3,6 tetrahydropyridine (MPTP induced Parkinson’s disease model C57BL control mice. Methods C57BL mice injected with MPTP 30 mg kg-1 d-1, row 5 d, Parkinson’s disease model mice. observe the HJD of mouse limb movement (climbing pole, hanging, swimming function, HPLC ECD brain striatum was detected Dopamine (DA and its metabolites dihydroxyphenylacetic acid (DOPAC, homovanillic acid (HVA content in mice brain tissue spectrophotometer superoxide dismutase (SOD, glutathione peroxidase (GSH Px ) and malondialdehyde (MDA) content. Results Compared with control group, model group were significantly lower extremity motor function (P lt;0.05, P lt;0.01, the striatum of DA, DOPAC and HVA were significantly reduced (P lt; 0.01), brain tissue SOD and GSH Px activity decreased, but the MDA content was significantly higher (P lt;0.01), HJD motor function in mice brain tissue loss and metabolic abnormalities have different degrees of regulation and improvement. Conclusion HJD of MPTP induced Parkinson’s disease in mice has a preventive effect. [Keywords:] HJD, Parkinson’s disease, MPTP, dopamine [Abstract] Objective To observe the protective effect of Huanglian Jiedu decoction (HJD) on mice with Parkinson’s disease (PD) induced by 1 methyl 4 phenyl 1, 2, 3, 6 tetrahydropyridine (MPTP). Methods 45 male C57BL mice were evenly randomized into 3 groups: control, MPTP model (subcutaneous injection 30 mg kg-1 d-1 for 5 days) and HDJ + MPTP groups (17.55 g crude drug / kg). HJD was given for 16 days by2intragastric administration and subcutaneous injection 30 mg / kg for 5 days at the eighth day. Performance of animals in the pole, traction and swimming test were observed at 4 days after the last injection. Levels of dopamin