MRN complex genes in the treatment of head and neck cancer overview.doc

 PAGE \* MERGEFORMAT 11 MRN complex genes in the treatment of head and neck cancer overview [Keywords:] MRN complex, DNA double-strand breaks, Radiosensitivity MRN complex is the Mre11, Rad50 and Nbs1 subunits composed of a highly conserved protein complex, is the first to recognize and signal DNA double-strand break response to child. Recent studies confirm that: it is double-strand breaks in DNA (Double Strains Breaks, DSBs repair process, plays a crucial role [1,2]. 1.MRN the basic structure of complex MRN complex in the 3 subunit protein containing multiple domains, ensuring that it is the reaction of DSBs, with architectural, structural study on the enzyme, feel and function of signal transduction [3] . MRN complex formation through the direct effect of Mre11 endonuclease will Nbs1, Rad50 and DNA bind together. Rad50 is a stable maintenance of chromosome structure proteins, enzymes containing the ATP binding box ATP (ATP binding cassette ATPase , zinc hook (Zn hook, and coiled coil (coiled coils structure. Through these structures, on both sides of bridge DNA ends, and easy of Mre11 for DNA end processing. the Nbs1 subunit N terminal contains FHA (forkhead associated domain) structure domain and BRCT (C terminal of a breast cancer susceptibility protein) domain, and its C terminal with ATM kinase (ataxia telangiectasia mutated kinase) reaction with the Mre11 interaction domain and so on. through these domains, the MRN complex to play a functional role . 2.MRN the role of the basic functions of complex In the process of repairing DSBs, MRN complex at least three major interrelated functions: quickly identify protein DNA DSBs and the establishment of a fixed framework, DSBs start point of identification and prosecution of the cell cycle signal transduction networks, and ATM kinase by DSBs outside the establishment identification, regulate the remodeling of chromatin around DSBs. MRN complex enable homologous recombination and non-homolog