Mushroom polysaccharide on acute liver injury in rats.doc

 PAGE \* MERGEFORMAT 14 Mushroom polysaccharide on acute liver injury in rats Of: Li Yifang, has Huai Wei, Wang Min, He Rongrong, Kurihara Bo [Abstract] Objective To investigate the mushroom polysaccharide (polysaccharides of Flammulina velutipes, FVP) on CCl4-induced acute liver injury in rats. Methods CCl4 mice by intraperitoneal injection of olive oil solution (0.5%, 5 mL kg-1) 12,24 , 48,72 h after the serum ALT, CCl4 acute liver injury to determine the best time for modeling 24 h. FVP (75,150,300 mg kg-1) continuous oral administration of 8 d, 7 d acute chemical preparation by CCl4 liver injury model, 24 h after the determination of FVP on serum ALT, AST activity, SOD activity of liver homogenate, MDA content. The results FVP can significantly reduce the CCl4 induced acute liver injury in mice serum ALT, AST activity and increased liver homogenate SOD activity and lower MDA content. Conclusions mushroom polysaccharide stress on CCl4 induced liver injury in mice a protective effect. [Keywords:] mushroom polysaccharides (FVP); liver injury; CCl4; ALT; AST; MDA; SOD Abstract: Objective The study was designed to investigate the protective effect of polysaccharides of Flammulina velutipes (FVP) against liver injury induced by CCl4. Methods When mice were intraperitoneally injected with olive solution (0.5%, 5 mL kg-1) for 12, 24,48,72 h, the plasma ALT activities were determined and the best time for CCl4 induced acute liver injury in mice was 24 h. FVP (75,150 and 300 mg kg-1) were orally administered to mice in experimental groups once per day for 8 days. On the seventh day, all mice were intraperitoneally injected with CCl4 to induce acute liver injury. 24 hours later, all mice were sacrificed, and blood and liver were collected for measurements of ALT and AST levels, the content of MDA and the activities of SOD. Results Oral administration with FVP significantly reduced the elevated levels of ALT and AST in mice with CCl4 induced acute liver