Mushroom snake inhibition endophyte and activity of active ingredients_0.doc

 PAGE \* MERGEFORMAT 9 Mushroom snake inhibition endophyte and activity of active ingredients Of: Zhang Yaxiong, Tu Xuan, Hu Bin, Deng Yixi, Yu Chen [Abstract] Objective To study the snake mushroom endophytes antibacterial activity of active ingredients. Methods of separation and purification of the 49 Balanophora involucrata endophytic fungi were purified using liquid fermentation broth extracted with ethyl acetate and butanol, and phase of ethyl acetate, butanol and aqueous phase were studied by Oxford, snake cup mushroom broth for the bacteria Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa antimicrobial effect. Results 18 endophyte strains snake mushroom broth of bacteria have antibacterial effects. antimicrobial active substance in the ethyl acetate phase. by thin layer chromatography and chemical reaction of ethyl acetate phase substances derived flavonoids and steroids. Conclusions isolated 18 strains of bacteria within the snake mushroom broth extracted with ethyl acetate phase flavonoids and steroidal have antibacterial effects. [Keywords:] Balanophora involucrata; endophytes; inhibition Balanophora involucrata Balanophora involucrata Hook. F., also known as deer grass jelly snake mushroom mushroom snake families are parasitic herbs, authentic traditional Chinese medicine in China, with a cooling blood to stop bleeding, the function of detoxification, attending Hyperactivity cough , hematemesis, intestinal wind of blood, the wind Ban heat rash, back pain, swollen boils and other drugs, people also used to treat hepatitis, cancer and other diseases, the traditional ethnic medicine, China has abundant resources. isolated endophytes mushroom snake and its metabolites were studied to find from the microbiological point of view of its active compounds, for the development of new drugs to open up new ideas. The subject of the distribution of snakes in the mushroom Simao Shennongjia object for the study of endoph