Nasal NK - T-cell lymphoma immunophenotype EBV infection and detection of gene rearrangements TCRγ.doc

 PAGE \* MERGEFORMAT 15 Nasal NK / T-cell lymphoma immunophenotype EBV infection and detection of gene rearrangements TCRγ Author: Liu prosperity, Zhong-Ling, XIONG Xiao-Liang, Zhu Jing, En-lin, gentle 【Abstract】 Objective To determine the nasal NK / T cell lymphoma (NK / TCL) immune phenotype, EBV infection and TCRγ gene rearrangement, in order to provide a basis for diagnosis and differential diagnosis. Methods Patients with diagnosis of nasal NK/TCL48 cases of paraffin-embedded specimens, using immunohistochemical SP labeling of LCA, CD79α, CD20, CD56, CD3, CD45RO, and EBV antibody to study its immune phenotype; EBER in situ hybridization detection of EBV-encoded small-molecule RNA (EBER); polymerase chain reaction amplification to detect TCRγ gene rearrangements. Results of 48 cases of nasal NK / TCL are expressed LCA, CD3, CD45RO, CD56, and EBV-positive rates were 44%, 52%, 73% and 19%, CD79α, and CD20 were negative; EBER-positive rate was 81%; TCR γ gene rearrangement positive rate was 19%. Conclusion nasal NK / TCL immune phenotype is inconsistent, not all cases of CD56 positive, CD3-positive in paraffin sections located in the cytoplasm; EBER is highly expressed in tumor cells, suggesting that they may have for the NK cell-derived; part of the TCR γ gene rearrangement-positive cases should be nasal NK-like T-cell lymphoma. Keywords: nasal NK / T cell lymphoma; Immunohistochemistry; in situ hybridization; polymerase chain reaction Immunophenotype and Epstein  Barr Virus Infection and Detection Rearrangement of T Cell Receptor γ (TCRγ) Gene in Nasal NK / T  cell Lymphoma Abstract: Objective To explore the immunophenotype and Epstein  Barr Virus (EBV) infection of nasal NK / T cell lymphoma and the significance of detection rearrangement of TCR γ gene in diagnosis and differential diagnosis of nasal NK / T cell lymphoma. MethodsFouty  eight cases of nasal NK / T cell lymphoma were studied, immunophenotype was analyzed