Nbn gene after birth and development of mouse hippocampus.doc

 PAGE \* MERGEFORMAT 13 Nbn gene after birth and development of mouse hippocampus [Abstract] Objective Nbn by observing the neural-specific gene knockout (Nbn CNS Del) and control (Nbn CNS Ctr) Development of morphological changes in hippocampus of mice to explore the DNA repair gene Nbn after birth in the hippocampus during development . Methods serial sections by light microscopy and stereological methods after birth (postnatal day, P) 7,14,21 d of the experimental and control groups were systematically hippocampal morphology and quantitative analysis. Results Nbn CNS Del hippocampal development has lagged far behind than Nbn CNS Ctr to P21d vertebral body layer and the dentate gyrus (DG) granular layer of the cross-sectional area were decreased (P lt;0.05), DG granular layer and polymorphic layer and the CA4 region density differences were statistically significant (P lt;0.05). Conclusion Nbn DNA repair gene is the mouse hippocampus is an important development genes, vertebral level of hippocampal granule cells mature and DG development and maturity. [Keywords:] Nbn, mice, hippocampal development, stereology ABSTRACT: Objective To investigate the role of neuro specific DNA repair gene Nbn in mouse hippocampus development by observing the morphological differences in Nbn CNS Del and Nbn CNS Ctr mice. Methods Serial sections and stereology were used to quantitatively analyze the development of mouse hippocampus on postnatal day 7, 14 and 21. Results Compared with that of the control group, the hippocampus development of Nbn CNS Del mice lagged behind. In Nbn CNS Del mice, on day 21, the profile area of pyramidal layer of hippocampus and granular layer of dentate gyrus decreased (P lt;0.05), and the volume densities in granular layer and polymorphic layer of dentate gyrus, and CA4 had significant differences (P lt;0.05). Conclusion DNA repair gene Nbn, an important gene in mouse hippocampus development, affects the development and maturation of hippoca