Narrow-band UVB on keratinocyte heparin-binding epidermal growth factor-like growth factor-mRNA of.doc

 PAGE \* MERGEFORMAT 8 Narrow-band UVB on keratinocyte heparin-binding epidermal growth factor-like growth factor-mRNA of Author: Russell Ju, Zhen-Hui Peng, Zheng Yan, Zhang Lu Kun, Guo-Rong Wang, Xu Hao Xiang [Abstract] Objective To study the narrow-band ultraviolet B (NB  UVB) on cultured normal human keratinocytes (KC) proliferation and heparin-binding epidermal growth factor-like growth factor (HB  EGF) mRNA expression. Methods 50mJ/cm2 and 100mJ/cm2 NB  UVB irradiated normal human KC to continue to foster after 12h, using MTT method and real-time quantitative RT  PCR method to detect KC proliferation and HB  EGF mRNA changes. Results NB  UVB irradiation normal KC, the KC may be a dose-dependent inhibition of proliferation and increase HB  EGF mRNA expression. 50mJ/cm2 and 100mJ/cm2 NB  UVB irradiation, respectively, so that KC inhibited the proliferation of 5.4% and 8.7%, so that HB  EGF mRNA increased to the normal control group, 1.7 times and 2.5 times. Conclusion NB  UVB inhibit KC proliferation in part through up-regulating the expression of HB  EGF-mediated. [Keywords:] epidermal growth factor; keratinocytes; narrow-band ultraviolet B In the subtropical rare in psoriasis, and psoriasis with emphasis on summer and winter and light features. This phenomenon prompted the temperature and sunlight may be related to the pathogenesis of psoriasis. Since the 20th century, 70 years, the clinical application of psoralen  began long-wave ultraviolet (psoralens plus ultraviolet A, PUVA) treatment of psoriasis, vitiligo and other skin diseases, followed by UVB (ultraviolet B, UVB) instead of UVA. The study found around 310nm of UV on the best treatment of psoriasis, redness effect was relatively small. Therefore, the filtering of other wavelengths generated by UV wavelengths of about 311nm narrow-band UVB (narrow  band UVB, NB  UVB) can be produced and widely used in skin treatment. Has proved to be NB  UVB treatment of psoriasi