Naringenin on thrombin-induced platelet aggregation of rabbit_0.doc

 PAGE \* MERGEFORMAT 6 Naringenin on thrombin-induced platelet aggregation of rabbit [Abstract] Objective To study the effect of naringenin on rabbit platelet aggregation induced by thrombin. Methods platelet suspension were incubated with naringenin 5 min, then thrombin (500 U / L) effect of 1 min, with a NIKON TE2000-E platelet aggregation observed fluorescence microscope. Results of naringenin on rabbit platelet aggregation induced by thrombin inhibited when the concentration of naringenin were 80,160,320 and 640 μmol / L, the inhibition of platelet aggregation increases with the concentration large and enhanced. Conclusion Naringenin has anti-platelet aggregation, and the intensity of the role of a positive correlation with the concentration of naringenin. [Keywords:] naringenin; platelet aggregation; thrombin Naringenin (naringenin, NGEN), is from citrus fruits, tea seeds, plants such as Changbai Stellera the extraction of natural bitter compounds [1]. In recent years, research on the growing number of naringenin and found it has a variety of pharmacological activities [2]. and naringenin is also play a role in platelet aggregation, platelet aggregation method we use, the use of thrombin as inducer, with the inverted fluorescence microscope naringenin on rabbit platelet aggregation induced by thrombin impact. now the results reported below. 1 Materials and equipment 1.1 Animals rabbits 4, ♂ ♀ use either body mass (2.0 + -0.2) kg, by the Guangdong Medical Animal Center. 1.2 Drugs and reagents naringenin, thrombin, N-2-hydroxyethyl piperazine-N ‘-2 - B acid (Hepes) were purchased from Sigma. The rest is pure analytical grade or biochemical reagents. 1.3 Instrument NIKON TE2000-E inverted fluorescence microscope, Germany. 2 Methods Reference [3]: the collection of rabbit blood and the use of plastic ACD (86 mmol / L sodium citrate, 111 mmol / L glucose, 53 mmol / L citric acid) 0.15 volume ratio of anticoagulation, 800r/min centrifu