Novel human chemokine-like factor superfamily 8 on human leukemia cell proliferation and epidermal growth factor receptor expression in.doc

 PAGE \* MERGEFORMAT 20 Novel human chemokine-like factor superfamily 8 on human leukemia cell proliferation and epidermal growth factor receptor expression in Author: Chen Shaoqian, Elaeocarpus, Huang Yumin, Wang Xin, Guqiao Li, DONG Zi-ming Abstract This study investigated the novel chemokine CKLFSF8 on human leukemia HL  60 cells proliferation and epidermal growth factor receptor (EGFR) expression, application of RT  PCR to detect CKLFSF8 gene in HL  60 cells; the use of liposome CKLFSF8 gene was transfected into HL  60 cells, cell growth was observed under inverted microscope; MTT cell proliferation assay level; CKLFSF8 immunocytochemistry to detect gene transfection HL  60 cells before and after the expression of EGFR. The results showed that: In the cell growth process can be detected CKLFSF8 expression; CKLFSF8 before and after gene transfection HL  60 cells were obviously inhibited the proliferation, and blank control group and P group were significantly difference (P lt;0.05 ); contrast to transfected cells before and after the positive rate of EGFR expression was significant difference (P lt;0.01). Conclusion: CKLFSF8 gene transfection on leukemia cells HL  60 proliferation and significantly inhibited the expression of EGFR. Keywords: CKLFSF8; HL  60 cells; cell proliferation; EGFR Effects of Novel Human Chemokine  like Factor Superfamily 8 on Proliferation and EGFR Expression of HL  60 Cells Abstract This study was aimed to investigate the effect of chemokine  like factor superfamily 8 (CKLFSF8) on proliferation and expression of epidermal growth factor receptor (EGFR) of HL  60 cells. Expression of CKLFSF8 mRNA on HL  60 cell line was assayed by RT  PCR; the target gene was transfected into the cells by lipid vector, cell proliferation was determined by MTT assay, while expression of EGFR in HL  60 was determined by immunocytochemical technique. The results indicated that expression of CKLFSF8 existed