NT4-Ant-Shepherdin [79-87] fusion gene recombinant vector.doc

 PAGE \* MERGEFORMAT 12 NT4-Ant-Shepherdin [79-87] fusion gene recombinant vector Of: Tang Xiaojiang, flat Baohua, Pan Chengen, Yang Guang laugh, Wang Quanying [Abstract] Design and Construction of the purpose of carrying NT4-Ant-Shepherdin [79-87] of the cDNA of the recombinant fusion gene vector for targeted therapy for Survivin basis. Methods asymmetric primer / template, PCR Preparation of NT4-Ant -Shepherdin [79-87] cDNA fragment, connected to pGEM-T-Easy vector, cloned and sequenced, digested connection with the PBV220/NT4 plasmid was transformed into competent cells E.coli DH5 @, was subcloned to obtain NT4-Ant- Shepherdin [79-87] fusion gene. Results cloned Ant-Shepherdin [79-87] genes, by restriction enzyme digestion and sequencing confirmed the results are correct, the connection PBV220/NT4, by cloning, restriction digestion, agarose gel electrophoresis confirmed that obtained 321 bp of the NT4-Ant-Shepherdin [79-87] The target gene fragments. Conclusion The success of molecular biology techniques constructed to carry NT4-Ant-Shepherdin [79-87] The recombinant fusion vector for further study targeting Survivin laid the foundation for anti-tumor effect [Keywords:] Survivin, Ant, neurotrophin 4 (NT4), fusion gene, vector ABSTRACT: Objective To investigate survivin as an anticancer therapeutic target by use of shepherdin [79-87], a novel peptide carrying the survivin sequence from Lys-79 through Leu-87, we constructed an recombinant vector containing fusion gene NT4-Ant-Shepherdin [79-87]. Methods The gene of Ant-Shepherdin [79-87] was obtained by PCR and T-vector method. After cloned and digested with restricted enzyme, Ant-shepherdin [79-87] was inserted in PBV220/NT4 vector. The recombinant vector was transformed into the competent cell, E.coli DH5 @. The fusion gene of NT4-Ant-Shepherdin [79-87] was identified by agarose gel electrophoresis (AGE). Results DNA sequencing results verified that the sequence of Ant-Shepherdin [79-87] was consist