Of different doses of insulin on serum IL early stage in severely scalded rats.docVIP

 PAGE \* MERGEFORMAT 2 Of different doses of insulin on serum IL early stage in severely scalded rats Author: Wang Yun-chuan, the peak, Jia Chi-yu, Zhang Wanfu, Tang Chao-Wu, HU Da-Hai [Keywords:] Burn; insulin; inflammation; Cytokines Regulating effects of different doses of insulin on serum IL1β and IL10 in early stage in severely scalded rats [Abstract] AIM: To determine the possible effects of different doses of insulin on the proinflammatory cytokine IL1β and the antiinflammatory cytokine IL10 in early stage of severe burn in rats. METHODS: Male SpragueDawly rats (30% TBSA, full thickness burn) were randomly divided into 2 groups to receive either normal saline only (n = 14, with intraperitoneal injection of 40 mL / kg normal saline, group A) or normal saline plus different doses of insulin (n = 42, with intraperitoneal injection of 40 mL / kg normal saline plus subcutaneous injection of 1, 3 or 5 IU / kg insulin respectively, group B1, B2, B3). In addition, 7 rats without thermal injury were subjected to normal control group (group N). The outcome measures encompassed the serum glucose, blood pressure, serum IL1β and IL10 in rats at 6 and 24 h after scald and different treatments. RESULTS: The serum glucose increased quickly in rats after scald, which was demonstrated in group A to a range of (10.7 ± 1.8) mmol / L by 6 h postscald. Insulin decreased the serum glucose in a dosedependent manner in rats of group B1, B2 and B3. And insulin at a dose of 3 IU / kg body weight reduced the serum glucose to a range of (6.0 ± 0.8 ) mmol / L in rats at 6 h after thermal injury, which was almost eqeal to the normal range of (5.1 ± 0.6) mmol / L (P