Oleanolic acid on cisplatin-resistant gastric cancer SGC7901 cell proliferation and apoptosis.doc

 PAGE \* MERGEFORMAT 17 Oleanolic acid on cisplatin-resistant gastric cancer SGC7901 cell proliferation and apoptosis Of: Li Hongmei Wang Jingchun Qizhan Peng Zhou Li Xing Guihua Liu Jicheng [Abstract] Objective oleanolic acid (OA) on cisplatin resistance of gastric cancer SGC 7901 (SGC 7901/CDDP) cell proliferation and apoptosis. Methods in vitro induced by cisplatin-resistant human gastric cancer cell line SGC 7901 / CDDP, MTT colorimetric assay SGC 7901/CDDP OA on cell proliferation; agarose gel electrophoresis of genomic DNA degradation; protein by Western blot the activation of apoptosis-related protease. Results MTT proved OA on SGC 7901 / CDDP inhibited the growth of cells, 100 mol / L OA 72 h, the inhibition of SGC 7901/CDDP rate of 62%, and showed time and dose dependent; agarose gel electrophoresis experiments show that 100 mol / L of OA processing SGC 7901/CDDP cells 48 h post-genomic DNA was degraded, showing a more typical DNA ladder; Western blot analysis showed that, 100 mol / L of OA processing SGC 7901/CDDP cells after 48 h, cell apoptosis protease caspase 3 is activated. Conclusion OA can induce SGC 7901/CDDP in vitro apoptosis, induction of apoptosis pathway may be the mitochondrial pathway. [Keywords:] oleanolic acid; SGC 7901/CDDP; cell proliferation; cell apoptosis Most of the current surgical treatment of gastric cancer is in advanced phases, and surgery alone is poor. As an important component of comprehensive treatment of chemotherapy, has become an important means of treatment of gastric cancer, more effective drugs are 5-fluorouracil, cisplatin (CDDP), ADM (1) and so on. apoptosis pathway defects are considered abnormal proliferation of malignant cells and the main reason for drug resistance to chemotherapy (2), thus inducing apoptosis of tumor cells has become an effective way to treat cancer. oleanolic acid (Oleanolic Acid, OA) is a natural drug, widely exist in plants, as free or combined into the form of glycosides. In