Olfactory ensheathing cell transplantation in rats after spinal cord hemisection ciliary neurotrophic factor and FGF2 expression in.doc

 PAGE \* MERGEFORMAT 21 Olfactory ensheathing cell transplantation in rats after spinal cord hemisection ciliary neurotrophic factor and FGF2 expression in Study: Chapter Bi Wenjie Wang Jian Ma Yuqiong Cheng Ting-Hua Wang Zhen-Dong Zhu Xiang ZHOU Xue [Abstract] Objective: To study the olfactory ensheathing cells (olfactory ensheathing cells, OECs transplanted into injured spinal cord, the ability to secrete ciliary neurotrophic factor (CNTF and basic fibroblast growth factor (FGF 2, and whether they can cause spinal cord Expression of CNTF and FGF 2 in change and how the number of cells in the spinal cord changes. Methods: Isolation and Culture of rat olfactory ensheathing cells, by immunofluorescence staining to detect the expression of CNTF and FGF 2. the olfactory ensheathing cells transplanted into SD rats with spinal hemisection were observed after 7 days and 21 days of olfactory ensheathing cells in the survival of the spinal cord, and spinal cord tissue biopsies CNTF and FGF 2 immunofluorescence staining, positive cells were counted and calculated the percentage of positive area. Results: Olfactory ensheathing cells in vitro and after transplantation were able to express CNTF and FGF 2. compared with the no transplant group, transplanted spinal cord CNTF and FGF 2 expression was significantly increased (P lt;0.05, neurons and increased number of oligodendrocytes (P lt;0.05), the number of astrocytes did not change significantly. Conclusions: Olfactory ensheathing cells into spinal cord injury can secrete CNTF and FGF 2, and to the spinal cord tissue expression of CNTF and FGF 2 increases, causing neurons and the number of oligodendrocytes increased. [Keywords:] spinal cord injury, olfactory ensheathing cells, transplantation, ciliary neurotrophic factor (CNTF, basic fibroblast growth factor (FGF 2 [Abstract] Objective: To investigate whether olfactory ensheathing cells (OECs) could secret ciliary neurotrophic factor (CNTF) and basic fibroblas