On guinea pig airway smooth muscle KATP channels in acutely isolated and recorded single channel currents.docVIP

 PAGE \* MERGEFORMAT 12 On guinea pig airway smooth muscle KATP channels in acutely isolated and recorded single channel currents [Abstract] Objective: To establish acutely isolated guinea pig airway smooth muscle of the method, and preliminary analysis of ATP-sensitive potassium channel (KATP channel) single-channel current in nature. Methods: The acute isolated guinea pig bronchus 3 to 4, and with pronase E dispersed airway smooth muscle cells within the patch clamp technique for recording outside the type to study airway smooth muscle KATP channel single-channel electrical properties. Results: The success recorded was 112.4 + -5.14 pS conductance, may be lower priority KATP channels are blocked carbohydrate single-channel current. clamping voltage between 0 ~ -60 mV, the channel current without rectification phenomenon, and no time-dependent inactivation. Conclusion: The acute separation method of airway smooth muscle and KATP channel single-channel current record of success for further study of airway smooth muscle KATP channel in respiratory diseases provide the basis for the role. [Keywords:] airway smooth muscle; acute isolation; ATP-sensitive potassium channel; single channel current The main role of airway smooth muscle to regulate airway tone and caliber of [1]. Airway smooth muscle contraction of respiratory diseases increased airway resistance in one of the main. A variety of ATP-sensitive potassium channel (ATP sensitive potassium channel, referred to as the KATP channel) opener, such as pinacidil (pinacidil) [2], cromakalim (cromakalim) [3], can make the airway smooth muscle relaxation, and this effect could be specific KATP channel blocker blocked by glibenclamide. Therefore, KATP channels in the regulation of airway smooth muscle relaxation and contraction play an important role, but the single-channel electrical properties has not been elucidated. The improved separation of airway smooth muscle cells isolated and with the patch clamp t