On High Performance Liquid Chromatography Huoxuetongluo content syringin Pill_0.docVIP

 PAGE \* MERGEFORMAT 9 On High Performance Liquid Chromatography Huoxuetongluo content syringin Pill [Abstract] Objective To establish Huoxuetongluo syringin Pill determination. Methods using high performance liquid chromatography column was Kromasil C18 (250 mm × 4.6 mm, 5 μm), mobile phase of acetonitrile -1% glacial acetic acid (10:90), flow rate 1.0 mL / min, detection wavelength was 270 nm, column temperature was at room temperature, the injection volume was 20 μL. Results syringin concentration 5.3 ~ 53 μg / mL a good linear relationship, The average recovery was 98.29%, RSD = 1.71%. Conclusion The method is simple, accurate, reproducible, and can be used as Huoxuetongluo quality control pills. [Keywords:] Huoxuetongluo pills, high performance liquid chromatography, syringin, determination Abstract: Objective To establish a HPLC method for the content determination of syringin in Huoxue Tongluo Pill. Methods Chromatographic assay was performed on Kromasil C18 column (250 mm × 4.6 mm, 5 μm) at room temperature, and the mixture solution of acetonitrile-1 % aqueous acetic acid (10:90, v / v) was used as mobile phase. The flow rate was 1.0 mL / min. The detection wavelength was at 270 nm. The sample size was 20 μL. Results Good linear relationship was achieved when the concentration of syringin was in the range of 5.3 ~ 53 μg / mL. The average recovery was 98.29% with RSD = 1.71%. Conclusion The method is simple, accurate and the results are stable with good reproducibility. This method can be used for the content determination of syringin in Huoxue Tongluo Pill. Keywords:: Huoxue Tongluo Pill, HPLC, syringin, content determination Huoxuetongluo Pills Chinese Medicine Hospital, Department of Wuwei Liangzhou developed after years of clinical experience in hospital pharmacy from Salvia, founder Ma, Millettia, frankincense, myrrh, astragalus and other herbs consisting of 11 traditional Chinese medicine, with phlegm stasis, pain in the function o