On RP-HPLC method for the determination of vascular health content of rutin in teabag.docVIP

 PAGE \* MERGEFORMAT 7 On RP-HPLC method for the determination of vascular health content of rutin in teabag Of: Luo Yanmei, coke is spent, Mao Zhi Chao [Abstract] Objective To determine the content of rutin in vascular health teabag method. Methods RP-HPLC (RP-HPLC), octadecyl bonded silica gel column separation of rutin with methanol - water - acetic acid (40:60:1) as mobile phase, flow rate 1.0 mL / min, detection wavelength 257 nm. Results The linear range of rutin 0.06 ~ 0.3 μg, r = 0.999 2, the average recovery was 98.48%, RSD = 0.87% (n = 5). Conclusion The method is convenient, sensitive, accurate, reproducible, and can be used for quality control of the product. Keywords:] vascular health teabag, rutin, RP-HPLC Abstract: Objective To establish a method for determination of rutin in Xueguankang Tea. Methods RP-HPLC method and C18 column were used for determination. Methanol-water-acetic acid (40:60:1) was the mobile phase with a flow rate of 1.0 mL / min. The detection wavelength was set at 257 nm. Results There was a good linear relationship of rutin within the range of 0.06 ~ 0.3 μg with r = 0.999 2. The average recovery of rutin was 98.48% with RSD = 0.87% (n = 5). Conclusion This method is convenient, sensitive, accurate and reproducible, and can be used for the quality control of Xueguankang Tea. Keywords:: Xueguankang Tea, rutin, RP-HPLC Vascular health teabag by the bud of other traditional Chinese medicine development, with cool blood to stop bleeding, liver blood level of effect, can reduce vascular fragility and restore blood vessel elasticity, free radical scavenging. Clinically for the treatment of arteriosclerosis, stroke, cardiovascular and cerebrovascular diseases, and as an auxiliary treatment of hypertension. Huaimi as Fangzhong Jun drug rutin as bud of the main active component [1]. for the effective control of the intrinsic quality of the product, the experimental use of anti- phase high performance liquid chromatogr