On the HPLC method for the determination OsteoKing hesperidin tablets_0.docVIP

 PAGE \* MERGEFORMAT 8 On the HPLC method for the determination OsteoKing hesperidin tablets [Abstract] Objective To establish OsteoKing to test the content of hesperidin tablets. Methods using high performance liquid chromatography, Shim-pack-C18 column (150 mm × 4.6 mm, 5 μm), acetonitrile -0.4 % phosphoric acid (20:80) as mobile phase, detection wavelength 283 nm. The linear range of the results of hesperidin 0.194 ~ 0.970 μg, r = 0.999 9, the average recovery of hesperidin was 98.50%, RSD = 0.63%. Conclusion The method for the determination of the film OsteoKing Hesperidin content, a simple, accurate, sensitive, and can be used as indicators of the quality control of the preparation. [Keywords:] OsteoKing film, hesperidin, HPLC, determination Abstract: Objective To determine the content of Hesperidin in Henggu Gushangyu Tablets by high performance liquid chromatography. Methods The determination was conducted on Shim-pack-C18 column (150 mm × 4.6 mm, 5 μm) with mobile phase of acetonitrile-0.4% phosphoric acid water (20:80). The detection wavelength was 283 nm. Results Good linear relationship of Hesperidin between area and amount was noted for 0.194 ~ 0.970 μg, with a correlation coefficient of 0.999 9. The average recovery was 98.50%, and RSD was 0.63%. Conclusion The established method is simple, accurate and sensitive, and can be applied to the quality control of the preparation. Keywords:: Henggu Gushangyu Tablets, Hesperidin, HPLC; content determination OsteoKing film is constant by the medicine of ancient bone healing agent (closing contained in the lt;”State standards for compilation of orthopedic medicine Volumegt;gt;) made changes in formulations and new agents [1], Smell the orange peel, safflower, Panax composed of 9 herbs, Qi with blood, liver or kidney, bone continued reinforcement, swelling and pain, promote healing effect for fresh fractures and delayed fractures and femoral head necrosis, bone joint disease, lumbar disc ps