On the impact of enzyme-linked immunosorbent assay results of common factors and control methods.docVIP

 PAGE \* MERGEFORMAT 11 On the impact of enzyme-linked immunosorbent assay results of common factors and control methods Author: Zhi-Ying Chen Tianhong Ying Ni [Paper Keywords] enzyme-linked immunosorbent assay; impact factor; control method Abstract: Enzyme-linked immunosorbent assay (enayme liked immunosorbent assay, ELISA) was developed in the 20th century, 70 years a kind of detection technology, because of their high sensitivity and specificity, simple operation, etc., are widely used in various kinds of antigens and antibodies, in order to support the clinical diagnosis and biological research has played a positive role in promoting. However, many factors influence the ELISA determination, the operation during each part of the test results will affect the error results. Is the author of the common factors that affect the experimental results and the corresponding analysis of the control method is as follows: A reagent element 1.1 The choice of reagents The choice of reagents is to ensure that the quality of key elements of the blood test. Although the countries have adopted the form of test Pipi ELISA reagents strict control, but the reagents used by different manufacturers are still in the differences in effect. To purchase a relatively high profile, with ‘three cards’ reagent, do not use non-batch of reagents, the best selection of reagents and instrument matching. More Do not use expired reagents and mix the different batches of reagents. 1.2 Reagent preparation In the clinical laboratory, for preparation of reagents in general do not pay attention, the usual practice is to experiment when the reagents from the refrigerator out to-use, while ignoring such an approach may affect the problem behind the lack of time, its direct consequences is the detection of some weak false-positive samples were negative. Therefore, ELISA determination of reagent preparation is the most critical, will start kit out the refrigerator, at room temperature 20 ~ 30 m