On the macroporous resin in purification of periwinkle and vincristine vinblastine_0.docVIP

 PAGE \* MERGEFORMAT 14 On the macroporous resin in purification of periwinkle and vincristine vinblastine Study: Pan Yuefeng, Wang Huimin, Zhang Yue, Han Jing, Zhang Lijun, Yan Limei, Yang Jing [Abstract] Objective To optimize the separation and purification periwinkle vinblastine and vincristine in the optimal process parameters. Methods of different types of macroporous resins roseus vinblastine and vincristine in the purified aqueous solution with different concentration of ethanol in different rate of elution, with silica gel column chromatography to HPLC trace detection. Results AB-8 resin separation good, 90% ethanol to 1.5 BV / h speed of elution than adsorption reached 74.5 mg / g, by silica gel column chromatography, the purity of 97.26% after the vincristine, vinblastine purity of 94.18%. Conclusions The process is simple and reasonable, good reproducibility, to get high purity of vinblastine and vincristine. Keywords:] macroporous resin, periwinkle, vincristine, vinblastine Abstract: Objective To optimize the technological parameters of separation and purification of vinblastine and vincristine from Gatharanthus Roseus. Methods Different types of macroporous adsorption resin were used to separate and purify vinblastine and vincristine from Gatharanthus Roseus, eluting with different concentration of alcohol aqueous and velocities, combined with silica gel column chromatography, the process was monitored by HPLC. Results AB-8 type resin showed better comprehensive adsorption property, 90% alcohol aqueous and 1.5 BV / h velocities were used to elute. Ratio adsorption quantity was achieved to 74.5 mg / g. Through silica gel column chromatography, the purity of vincristine was achieved to 97.26% and the purity of vinblastine was achieved to 94.18%. Conclusions The process is simple and reasonable with good reproducibility. It is effective to enrich highly purified vinblastine and vincristine. Keywords:: macroporous adsorption resin; Gatha