Phyllanthus cotyledons of qualitative and quantitative analysis of quercetin_0.doc

 PAGE \* MERGEFORMAT 10 Phyllanthus cotyledons of qualitative and quantitative analysis of quercetin Of: Zhongzhen Guo, Li Xuejian, Dong Jiao, Bu-Ming Liu, Chen Mingsheng, Lin Xiao, Wei Yanfei, Weiyong Han, Lu Shengsheng, Huangjin Lan, Luo Xue Fei, Zhong Yining [Abstract] Objective To analyze Phyllanthus cotyledons of quercetin, the establishment of qualitative and quantitative analysis methods, quality standards for the development of cotyledons Phyllanthus provide method and basis. Methods for qualitative identification by thin layer chromatography; than by liquid chromatography quercetin content in cotyledons Gan: The column was C18 column, mobile phase of methanol -0.2% phosphoric acid (50:50), flow rate 1.0 ml / min, detection wavelength was 360 nm, external standard. Results Quercetin In the 10 ~ 100 μg / ml linear range of the average recovery was 101.0% (RSD = 1.89%, n = 9); were samples of different origin were determined. Conclusion The method is simple, accurate and specific Strong, reproducibility, recovery, and can be used as quality control of medicinal Phyllanthus cotyledon method. [Keywords:] Phyllanthus cotyledons; quercetin; thin layer chromatography; liquid chromatography Euphorbiaceae Phyllanthus cotyledons genus Phyllanthus emblica Phyllanthus embllca L. leaves, Phyllanthus emblica tree widely distributed in China’s Guangxi, Guangdong, Fujian, Guizhou, Yunnan and other places. Emblica traditional ethnic medicine, with heat cooling blood, stomach and digestion, fluid cough effectiveness, for the treatment of blood, blood sedimentation, indigestion, abdominal distension, cough, sore throat, dry mouth [1]. emblica containing quercetin [2], but cotyledons of Phyllanthus not been reported. we found that Phyllanthus cotyledons also contain quercetin, the present study, thin layer chromatography and liquid chromatography, with quercetin as a control, cotyledons were of Phyllanthus qualitative and quantitative analysis method, and sa