Pinellia germplasm resources of random amplified polymorphic DNA technique.doc

 PAGE \* MERGEFORMAT 11 Pinellia germplasm resources of random amplified polymorphic DNA technique Study: Po Yang Jun, Zhu Xiu Zhi, Luo Branch, Li Jun, Weihui Ting, Zheng-Song Peng [Abstract] Objective To analyze Pinellia germplasm at the molecular level of genetic diversity. Method using random amplified polymorphic DNA technique (RAPD) of 21 tested Pinellia germplasm resources. The results were obtained from 29 primers 162 amplified DNA fragments were 123 (75.9%) polymorphic fragments, Pinellia populations revealed abundant genetic diversity. using UPGMA cluster analysis showed that all materials can be divided into three categories, based on RAPD groups of genetic similarity by a certain relationship with the geographic distribution. Conclusion ternata germplasm at the molecular level greater genetic differences do exist. RAPD markers can be used as DNA fingerprinting Pinellia build an effective tool. [Keywords:] Breit, random amplified polymorphic DNA technology, genetic diversity, DNA fingerprinting Abstract: Objective To discuss the genetic diversity of the germplasm resources of Pinellia ternata on molecular level. Methods The genetic diversity of P. ternata 21 different populations were tested by RAPD markers. Results A total of 162 products were amplified by 29 10-mer arbitrary primers, 123 (75.9%) products were found to be polymorphic. The results revealed the abundant genetic diversity in P. ternata 21 different populations. The cluster analysis using UPGMA method showed that 21 populations were classified as 3 groups, and the genetic diversity in P. ternata based on RAPD GS was correlated with geographic distribution. Conclusion There actually existed much genetic diversity on molecular level among the germplasm resources of P. ternata. RAPD markers could be effective tools to construct DNA fingerprintings of P. ternata. Keywords:: Pinellia ternata, RAPD, genetic diversity, DNA fingerprintings Ban Xia [Pinellia ternata (Thunb.) Bre