Polygonum cuspidatum resveratrol and resveratrol glucoside in the Extraction and Determination.docVIP

 PAGE \* MERGEFORMAT 8 Polygonum cuspidatum resveratrol and resveratrol glucoside in the Extraction and Determination Author: Liu-wang, Tengzeng Hui, ZHANG Bang-Le, Huan Meng Lei, Yang Qian, Thursday yuan [Abstract] Objective: To establish a simultaneous determination of resveratrol and resveratrol glucoside content of high-performance liquid chromatographic method to optimize the active ingredient in Polygonum cuspidatum resveratrol and resveratrol glucoside extraction process. Methods: To develop a gradient method Determination of resveratrol and resveratrol glycosides in high-performance liquid chromatography method to resveratrol and resveratrol glycosides total of the indexes, designed to ethanol concentration, solvent ratio and temperature for the ethanol extract of the four factors factor three-level orthogonal experiment to determine the active ingredients in Polygonum cuspidatum The optimum extraction. Results: In the selected chromatographic conditions, Polygonum cuspidatum extract resveratrol and resveratrol glucoside can achieve the baseline separation. detection of white - veratryl alcohol and its glycosides in the linear regression equations were Y = 0.0006X-0.183 (r = 0.9996), Y = 0.0011X-0.478 (r = 0.9993); linear in the range of 0.48 ~ 38.4 mg / L and 0.47 ~ 38.2 mg / L. day, day precision are less than 5%, recovery of 90% ~ 105%, and sample analysis time of less than 7 min. Polygonum cuspidatum active ingredient resveratrol and resveratrol glucoside The optimum extraction of 80% ethanol concentration, solvent multiples of 15, alcohol extraction temperature 70 ℃ , extraction time 2 h. Conclusion: The establishment of accurate and reliable, rapid analysis can detect resveratrol in Polygonum cuspidatum extract and white Veratrum glycosides analytical methods to optimize the extraction process of quality control. [Keywords:] Polygonum cuspidatum; resveratrol; resveratrol glycosides; high-performance liquid chromatography 0 Introduction Po