Pseudolaric acid on mice inoculated into the armpit of the sarcoma 180 cells in vitro.docVIP

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2017-05-05 发布于浙江
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Pseudolaric acid on mice inoculated into the armpit of the sarcoma 180 cells in vitro.doc

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Pseudolaric acid on mice inoculated into the armpit of the sarcoma 180 cells in vitro

Pseudolaric acid on mice inoculated into the armpit of the sarcoma 180 cells in vitro Author: Wang Ye Shaoli Zhu Sheng Zhang Hui Zhao Weicheng car light [Abstract] Objective To investigate the Pseudolaric acid (PAA) on the inoculated mice axillary subcutaneous sarcoma 180 (S180) cells in vivo. Method in mice inoculated subcutaneously with S180 cells in axillary, and then with different concentrations of PAA to the various groups of mice for intraperitoneal injection of 7 d. Continue to 5 d after feeding the mice were killed, saying the tumor weight, in the light and electron microscope observation of tumor tissues and development status. The results of anti-tumor effects of different concentrations of PAA are also different. Were slightly larger than the concentration of anti-tumor effect is good. PAA block of tumor growth, tumor cell volume and inhibit tumor cell division, etc., and can promote tumor cell apoptosis. Conclusion PAA could inhibit S180 mice inoculated into cell division and proliferation, promoting tumor cell apoptosis, and thus extending the life span in mice. [Keywords:] pseudolaric acid; sarcoma 180 cells; tumor cell apoptosis [Abstract] Objective To investigate the inhibitive effect of pseudolaric acid A (PAA) on sarcoma 180 (S180) cells inoculated into the hypoderm of mouse armpit. Method S180 cells were inoculated into the hypoderm of mouse armpit. Different concentration of PAA was then given to these mice for seven days by way of peritoneal cavity injection. Five days later, the mice were killed. The tumors were cut off and weighed, and made into sections for light microscope and electron microscope observation. Result The inhibitive effects of PAA on S180 cells were different due to different concentration of PAA given to the mice. The greater the concentration of PAA, the more obvious the inhibitive effect of it. Both mitosis and growth of the tumor cells were inhibited by PAA. Obvious apoptosis of tumor was seen under microscope. Con