Purification and Determination of Rutin.doc

 PAGE \* MERGEFORMAT 19 Purification and Determination of Rutin Author: Wen-Bin Zhao, Xu Yuhua, WANG Lu Shi, Dong-Min Mo 【Abstract】 Objective To prepare high-purity rutin was used as a reference substance for the purpose or to meet the special needs of other reagents. Method to extract rutin from Sophora japonica, and its preparation into a reference substance. Using both simple and economical to mention the acid alkaline precipitation Rutin extracted from Sophora japonica, after refinement, rutin reference substance was isolated, and used the general approach, thin-layer chromatography, nuclear magnetic resonance method identified HPLC determination of its content. The results by chemical method, were identified by thin-layer chromatography, nuclear magnetic resonance identification of that sample and rutin rutin standard plans are basically the same; high-performance liquid content of 98.05% measured. Conclusions The laboratory preparation method is simple rutin reference substances, the product of high purity can be used as reference substance used instead of standard. Keywords: rutin; separation; purification; high-performance liquid chromatography; determination Abstract: ObjectiveTo establish the methods for purification, identification and determination of rutin reference substance.Methods Extract rutin from Sophora japonica L. by the alkali extraction and acid precipitation. Then refinement and separation of the rutin reference substance was done. Identified the substance by the normal way. TLC and NMR. HPLC was used to determine the content of rutin. Results After identified by chemical way, TLC, and NMR, the results showed that the chart of the sample of rutin and the chart of standard substance were unanimous, the mean content was 98.05%. ConclusionThis way of preparing rutin in the lab is simple and practical. The products are very pure, so it can be used in place of substance replaced standard substance. Keywo