Qi Deficiency on the renal tissue of rats aquaporin 2 expression in_0.doc

 PAGE \* MERGEFORMAT 10 Qi Deficiency on the renal tissue of rats aquaporin 2 expression in Of: Wang Zhe, Sun Hongwei, Wang Hao, LIU Chun, in of the new, Wang Shan [Abstract] Objective To observe the renal tissue of rats Qi Deficiency aquaporin 2 (AQP2) expression changes, as the process of body fluid metabolism provide the basis for the theory of lung and kidney. Methods 20 rats were randomly divided into model group and control group, by immunohistochemistry, Western blot, RT-PCR method for determination of renal AQP2 protein and mRNA expression. Results Qi deficiency model group increased expression of renal AQP2, AQP2 mRNA expression increased, compared with normal control group, the difference was significance (Pgt; 0.01). Conclusion Qi Deficiency AQP2 expression in renal tissue of rats increased, suggesting that lung main “water passage” function may affect the expression of renal AQP2. Keywords:] Qi deficiency, kidney, aquaporin 2, rats Abstract: Objective To observe the effects of kidney aquaporin-2 (AQP2) expression in Lung-Qi deficiency model rats and provide experimental evidence for the traditional Chinese medical theory that the kidney is related with the lung in charge of regulating the water passage. Method Twenty healthy rats were divided into the model group and the control group. The method of immunohistochemistry and Western blot was used to determine the expression of AQP2 in kidney. RT-PCR was applied to analyze the expression of AQP2 mRNA in lung and kidney tissues. Result The expression of AQP2 and AQP2 mRNA in kidney in model group was higher than in the control group. Conclusion The expression of AQP2 in kidney was higher in Lung-Qi deficiency model rats. One of the substance basis of “lung in charge of regulating the water passage “may be related to the change of AQP2 expression in the kidney tissues. Keywords:: Lung-Qi deficiency, kidney tissues, aquaporin-2, rat Chinese medicine believes that “the main water kidne