Rabbit anti-human cytochrome P450 4A11 synthetic peptide antibodies and identification of.docVIP

 PAGE \* MERGEFORMAT 15 Rabbit anti-human cytochrome P450 4A11 synthetic peptide antibodies and identification of Authors: Yang Wei, LIU Hai-ying, Wu Zheng-Hua, Xiao-Bo Tang, Zhu Ling [Keywords:] CYP4A11;, synthetic peptide;, polyclonal antibody [Abstract] Objective: To prepare rabbit anti-human cytochrome P450 4A11 (CYP4A11) antibodies, and preliminary identification of its characteristics. Methods: Using bioinformatics to analyze CYP4A11 protein sequence, according to hydrophilic structure, flexibility area, antigen index and surface probability of the structure of the choice of indicators peptide sequence, synthetic peptides CYP4A11 with the carrier protein keyhole limpet hemocyanin (KLH) even Alliance, the immune rabbits were prepared by anti-CYP4A11 antibody. Purification of ammonium sulfate bitterness antibodies, the purified antibody ELISA, Western blot, immunohistochemical staining were identified. Results: The anti-CYP4A11 synthetic peptide antibodies. The antibody titer of 1:32000. Synthetic peptide with CYP4A11 and natural CYP4A11-specific reactions occur, the antibodies recognized the corresponding antigen relative molecular mass (Mr) of 53,000, identifiable normal liver CYP4A11. Conclusion: The synthetic peptide KLH complex is immunogenic, can be used to prepare the appropriate antibodies. Preparation of rabbit anti-CYP4A11 synthetic peptide antibodies can be used in ELISA, Western blot, immunohistochemistry experiments. [Keywords:] CYP4A11; synthetic peptide; polyclonal antibody Human cytochrome P450 4A11 is one of the cytochrome P450 4A isoforms, mainly in the liver and kidneys, which can be catalyzed a variety of fatty acids (lauric acid, etc.) metabolism, regulate lipid metabolism in the body balance [1]. CYP4A11 catalyzed arachidonic acid metabolism of endogenous generation of 20-hydroxy twenty arachidonic acid (20H ydroxyeicosatetraenoicacid, 20HETE), while the formation of 20HETE with essential hypertension [2]. CYP4A11 is also involved