Rabbit bone marrow stromal cells in vitro β-tricalcium phosphate composites for posterior thoracic spinal fusion experimental study.doc

 PAGE \* MERGEFORMAT 12 Rabbit bone marrow stromal cells in vitro β-tricalcium phosphate composites for posterior thoracic spinal fusion experimental study Author: Xu Jian-Xi Lu Lu Xuhua guard Jia-shun GU Hong-Lin Teng Tong Yang Jiandong Chen Changqing Keywords: spinal fusion Abstract: [Objective] To evaluate β-tricalcium phosphate bone marrow stromal stem cells for bone graft substitutes for spinal fusion effect. [Method] 60 rabbits divided into four groups thoracic posterior fusion: Ⅰ group (tissue-engineered bone that is β-TCP composite BMSCs), Ⅱ group (β-TCP + autologous red bone marrow), Ⅲ group (pure β-TCP), Ⅳ group (autogenous iliac bone). After 12 weeks the remaining animals were killed radiology, manual mechanical and histological examination. [Results] from the imaging and manual mechanical comparison, Ⅰ group fusion rate was higher than Ⅱ, Ⅲ, Ⅳ group and there is statistically significant. Histologically, Ⅰ group the amount of new bone formation are more filled with new bone within the porous ceramics; Ⅱ group compared with the amount of trabecular bone formation in group Ⅰ less for new bone and fibrous tissue mixed; Ⅲ group of porous ceramic bone particles within the multi - for the connective tissue, new bone less, I saw a small amount of new bone attached to the surface of ceramic bone; Ⅳ group lamina and transplantation of bone at the junction with sparse formation of new bone trabeculae. [Conclusion] β-TCP composite BMSCs spinal fusion with autogenous bone is a good substitute. Keywords: Bone marrow stromal stem cells; β-tricalcium phosphate; spinal fusion; bone graft substitutes Bone marrow stromalderived osteoblastsporous βtricalcium phosphate composites as bone graft substitute for posterior thoracic spinal fusion Abstract: [Objective] To evaluate the efficacy of autogenous bone marrow stromalderived osteoblastsporous (BMSCs) βtricalcium phosphate (βTCP) composites as an alternative to autogenous graft mater