Receptors in whole saliva in the detection of Streptococcus mutans.doc

 PAGE \* MERGEFORMAT 12 Receptors in whole saliva in the detection of Streptococcus mutans [Abstract] Objective factors of Streptococcus mutans adhesion protein and the interaction of salivary proteins, salivary Streptococcus mutans whole screen bonding hormone receptor component protein. Methods strains of Streptococcus mutans by the activation of international standards, liquid culture, the use of frozen ethanol Extraction of bacteria cultured in liquid medium secreted to the bond factor, and pigment composition adhesive gel electrophoresis, determination, and antigen detection. Select 15 and 15 caries-free caries were, respectively, after eating 2h Its non-irritating whole saliva collected by freeze-drying the sample after preparative electrophoresis, using SDS polyacrylamide gel electrophoresis (SDS PAGE) to separate the elements with a Streptococcus mutans adhesion protein and antibody components of Streptococcus mutans immune Western blotting blot. Results of bacterial culture broth of Streptococcus mutans to the surface bonding hormone secreted components. Western blotting showed that receptors of Streptococcus mutans in saliva of at least 6 components, not limited to the current understanding of protein-rich breast etc. 3. Conclusions Streptococcus mutans surface bonding elements with a variety of whole saliva protein binding, which may be Streptococcus mutans adhesion to the material basis of the tooth surface. [Keywords:] caries, Streptococcus mutans, saliva protein, adhesion, bonding hormone ABSTRACT: Objective To select the acceptor ingredients of Streptococcus mutans protein in the whole saliva through the interaction between adhesion and saliva protein of Streptococcus mutans. Methods After activation and liquid culture of Streptococcus mutans strains of international standards, we took Su bonding from liquid culture of bacteria secretion in the process of liquid culture by using chilled ethanol extraction. Then su bonded components under