Recombinant Echinococcus multi-Bb-Em14-3-3 Construction and expression efficiency of the vaccine.docVIP

 PAGE \* MERGEFORMAT 17 Recombinant Echinococcus multi-Bb-Em14-3-3 Construction and expression efficiency of the vaccine [Abstract] Objective To construct a multi-Echinococcus (Em) re-Bifidobacterium (Bb)-Em14-3-3 vaccines, and research Em14-3-3 molecule in E. coli BL21 (DE3) Expression efficiency. PCR amplification methods by Em14-3-3 antigen gene, the gene was cloned containing glutathione-S-transferase (GST) gene of Escherichia coli - Bifidobacterium shuttle vector pGEX-1T, The recombinant plasmid pGEX-Em14-3-3, electroporated the plasmid into Bb, build more recombinant Echinococcus