Recovery capsule on bone and cartilage cells and Bax expression of Bcl-2mRNA_0.docVIP

 PAGE \* MERGEFORMAT 13 Recovery capsule on bone and cartilage cells and Bax expression of Bcl-2mRNA [Abstract] Objective To observe the recovery capsule on rat model of knee osteoarthritis chondrocyte apoptosis related genes Bax and Bcl-2 and to explore the treatment of osteoarthritis in rats. Methods 48 male SD rats were randomly divided into normal group , model, Zhuanggu joint pill group, the recovery capsule group, 12 in each group. In addition to the normal group, other groups were established by Hulth animal models of knee osteoarthritis .4 weeks later, Zhuanggu joint pill group, the recovery capsule groups were given Zhuanggu joint pills, capsules orally recovery, while the normal group, model group received normal saline .8 weeks later, rat knee articular cartilage cell ultrastructure for electron microscopy, in situ end labeling (TUNEL method ) chondrocyte apoptosis detected by reverse transcription / polymerase chain reaction (RT-PCR) assay Bax and Bcl-2mRNA expression. The results of recovery capsule can inhibit excessive apoptosis of cartilage cells and reduce the pro-apoptotic gene Bax mRNA expression, increased apoptosis gene Bcl-2mRNA expression, and regulation of Bax/Bcl-2 ratio. Conclusion recovery capsule by reducing the expression of proapoptotic gene Bax mRNA, increased apoptosis gene Bcl-2mRNA expression, regulating Bax / the ratio of Bcl-2 inhibition of apoptosis in osteoarthritis chondrocytes. [Keywords:] knee osteoarthritis; apoptosis; recovery capsule; Bax; Bcl-2 Osteoarthritis (Osteoarthritis, OA) is a common clinical, seriously affecting the quality of life in the elderly is a chronic, progressive joint disease, articular cartilage degeneration is a key link in the pathogenesis of OA. Recent studies have reported that cartilage cells withered death in articular cartilage degeneration plays an important role [1,2]. recovery capsule is a clinical term for the treatment of osteoarthritis significant effect of Chinese herbal com