Red kidney bean hemagglutinin monomer Purification and Characterization of.doc

 PAGE \* MERGEFORMAT 14 Red kidney bean hemagglutinin monomer Purification and Characterization of [Abstract] Objective To study the extract from red kidney bean hemagglutinin monomers (PHA-E) of the new methods. Method of red kidney beans after extraction, ion exchange resin separation, molecular sieve chromatography purified samples obtained after PHA-E. Were determined using electrophoresis purity, molecular weight and isoelectric point. Cell suspension with 2% of people the ability to determine the sample agglutinate red blood cells and clotting factors that influence the use of sulfuric acid determination of their sugar content of phenol. Results PAGE analysis of PHA-E sample as a single band electrophoretically pure, SDSshow subunit molecular mass of 32 kD, and isoelectric point of 6.5. Samples of people 50% of agglutination of red blood cell protein Low concentration of 4 μ g / ml or so. Monosaccharide does not affect the PHA-E coagulation activity, EDTA inhibit its clotting activity, Zn2 promote its clotting. Sugar content of 8.1%. [Keywords:] hemagglutinin purified coagulation properties Purification and Partial Characterization of Erythrohemagglutinin from Phaseolus Vulgaris HE Tao1, ZHANG Tao2 *, WU Chang-ying1, WANG Liang1, SHAN Xiao-xue1, LUO Qin1 (School of Preclinical Medicine, Chengdu Medical College, 1.2006s Biotechnology, 2.Biotechnology laboratory, Chengdu, 610083, China) Abstract: Objective To study a new method for Erythrohemagglutinin (PHA-E) production from Phaseolus vulgaris.Methods PHA-E was purified from the excellent seeds of Phaseolus vulgaris by extraction, ion exchange chromatography and final gel filtration chromatography.The purity, the molecular weight and the isoelectric point of purified PHA-E were determined by electrophoresis.The ability and the influencing factor of agglutination were determined by 2% erythrocytes of human.Conclusion The purified PHA-E was single PAGE outline and had apparent subunit molecular weights