Reflux-induced Barrett.docVIP

 PAGE \* MERGEFORMAT 9 Reflux-induced Barrett Author: Zhang Tao Zhu-fang Xiao-Fei Zhang Feng Wang YJ Cheng QS [Keywords:] Barr Expression and significance of inducible nitric oxide synthase in formation of Barretts esophagus and its progression to esophageal adenocarcinoma in a rat model [Abstract] AIM: To investigate the expression and significance of inducible nitric oxide synthase (iNOS) in the formation of Barretts esophagus (BE) and its progression to esophageal adenocarcinoma (EAC) in a rat model. METHODS: Sixtyfive eightweekold male SpragueDawley rats underwent endtoside gastrojejunostomy, followed by endtoside esophagojejunostomy approximately 05 cm distal to the gastrojejunostoma to produce the duodenogastroesophageal reflux model group. Another 10 rats undergoing a sham operative procedure were taken as a control group. Some animals were sacrificed and their esophageal samples were taken from the model group at week 1, 4, 8, 12, 16 and 20 respectively after surgery and from the control group at week 20. Longitudinally esophageal paraffin slides were made and used for hematoxylin and eosin staining and iNOS immunohistochemical staining. Pathological changes and expression of iNOS in the distal onethird of the esophageal mucosa were detected microscopically. RESULTS: ① No pathological changes were found in the control group. Significant esophagitis were observed in the model group after surgery. There was a progression in epithelial cell proliferation and inflammation from mild to severe in the distal onethird of the esophagus, and BE and EAC were observed. The incidence of BE and EAC increased with time: 375% and 0% at week 8, 60% and 10% at week 12, 90% and 30% at week 16, 86.6% and 533% at week 20. ② Immunohistochemical staining of iNOS was negative in the control group. In the model group positive expression of iNOS in the distal onethird of the esophagus was observed in 0%, 0%, 50%, 80%, 100% and 100% of the rats at week 1, 4, 8, 12, 16 and 2