Resveratrol from Polygonum cuspidatum Extraction and Separation Technology.docVIP

 PAGE \* MERGEFORMAT 8 Resveratrol from Polygonum cuspidatum Extraction and Separation Technology Authors: XU Han-lin, FU Yi and, GAO Peng, XU Cheng-jun [Keywords:] Polygonum;,,,, resveratrol;,,,, macroporous resin;,,,, silica gel column chromatography Abstract: Objective To optimize the best extraction of Resveratrol in Polygonum cuspidatum. Methods ① orthogonal experimental method to extract the amount of resveratrol as an indicator, high performance liquid chromatography (HPLC) test content, optimal extraction conditions. ② The macroporous resin enrichment and chromatography separation, purification of resveratrol. The optimum results of ethanol extract of 10 times the amount of 90% ethanol was extracted two times, 1 h / time. The AB8 resin adsorption enrichment, silica gel column chromatography purification, high purity resveratrol products available. Conclusion The process can be used to guide production. Keywords:: Polygonum cuspidatum; Resveratrol; macroporous resin; silica gel column chromatography Study on Extraction Process of Resveratrol in Polygonum cuspidatum Abstract: ObjectiveTo study the optimum extraction process of resveratrol in Polygonum cuspidatum.Methods ① The optimum extraction process was investigated by orthogonal design and the index of the method was the contents of resveratrol. ② The technology of macroporous adsorptive resin and chromatography was used in the separation and purification of resveratrol by HPLC.ResultsThe optimum condition of ethanol extraction: 10 times amount of 90% ethanol and extracted 2 times, 1 hour one time.The purity of the product was gained by the technology of resin AB8 adsorption and silica column chromatography purification.ConclusionThe optimum extraction process can direct production. Keywords:: Polygonum cuspidatum; Resveratrol; Macroporous adsorptive resin; Slica column chromatography Resveratrol (3,4 ‘, 5, 3-hydroxy-stilbene; transresveratrol) are a class of lipophilic polyphenols, in t