RP-HPLC determination of ursolic acid and forsythia in the content of oleanolic acid.doc

 PAGE \* MERGEFORMAT 11 RP-HPLC determination of ursolic acid and forsythia in the content of oleanolic acid Authors: Sheng-Qin Zou, Yu Ji-hai, happy-hua, Chen Wu [Keywords:] forsythia;,, ursolic acid;,, oleanolic acid;,, high-performance liquid chromatography Abstract: Objective To establish simultaneous determination of ursolic acid and forsythia in the determination of oleanolic acid method. Methods using high performance liquid chromatography (HPLC) determination of its content. Column was NovaPak C18 (3.9 mm * 300 mm, 4 μ m), mobile phase was methanol-water (88:12), flow rate 0.8 ml / min, detection wavelength 210 nm, column temperature was 25 ℃ . Results Ursolic acid and oleanolic acid, respectively 0.912 ~ 4.560 μ g (r = 0.999 8) and 0.312 ~ 1.560 μ g (r = 0.999 6) within the peak area and sample volume showed a good linear relationship; ursolic acid and oleanolic acid, the average recoveries were 98.2% (RSD = 1.1%) and 97.8% (RSD = 1.4%). Conclusion This method is simple, convenient, fast, accurate and can forsythia quality evaluation and control of traditional Chinese medicine to provide a scientific basis. Keywords: Forsythia; Ursolic acid; oleanolic acid; high performance liquid chromatography HPLC Determination of the Contents of Ursolic Acid and Oleanolic Acid in Forsthia suspense (Thunb.) Vahl Abstract: ObjectiveTo develop a method for the quantitative analysis of ursolic acid and oleanolic acid in Forsythia suspense simultaneously.MethodsHPLC was carried out on the NovaPak C18 column (3.9 mm * 300 mm, 4 μ m) at 25 ℃ using MeOHH2O (88:12) as mobile phase with a flow rate of 0.8 μ l and detection wavelength was set at 210 nm. ResultsThere was good linear relationship between the peak area and the sample content injected at the ranges of 0.912 ~ 4.560μ g (r = 0.999 8) and 0.312 ~ 1.560 μ g (r = 0.9996) for ursolic acid and oleanolic acid respectively. The average recovery rate of ursolic acid and oleanolic acid were 98.2% (RSD 1.1%) and 97.8%