RP-HPLC method for the determination on the callus Chuanxiong ferulic acid.doc

 PAGE \* MERGEFORMAT 8 RP-HPLC method for the determination on the callus Chuanxiong ferulic acid [Abstract] Objective To establish a HPLC method for the determination of callus Chuanxiong method of ferulic acid. Methods ZORBAX 300SB-C18 (250 mm × 4.6 mm, 5 μm) column, acetonitrile -1% triethylamine ( 15:85) as mobile phase, detection wavelength was 320 nm, flow rate 1.0 mL / min, column temperature is 27 ℃, the injection volume was 20 μL. The results of ferulic acid in 0.93 ~ 4.67 μg / mL a good linear relationship, r = 0.999 8, the average recovery was 99.98%, RSD = 1.63%. Conclusion The method is simple, accurate and reliable for Chuanxiong callus determination of ferulic acid. [Keywords:] Chuanxiong, callus, ferulic acid, HPLC Abstract: Objective To establish a HPLC method for the determination of Ferulic acid in callus of Ligusticum chuanxiong. Methods The separation was performed on ZORBAX 300SB-C18 (250 mm × 4.6 mm, 5 μm) column, using acetonitrile-1% triethylamine (15 : 85) as mobile phase, detected at 320 nm. The flow rate was 1.0 mL / min, the column temperature was 27 ℃. Results The linear range of Ferulic acid was 0.93 ~ 4.67 μg / mL, r = 0.999 8, the average recovery was 99.98% and RSD was 1.63%. Conclusion The method was rapid, simple, accurate and stability, and can be used for the quantitiative determination of Ferulic acid in Ligusticum chuanxiong callus. Keywords:: Ligusticum chuanxiong, callus, Ferulic acid, HPLC Ferulic acid is a medicinal plant Chuanxiong (Ligusticum chuanxiong Hort.) Main components. We had Chuanxiong tissue culture and callus by HPLC method in the determination of ferulic acid, which is produced by cell engineering approach ferulic acid to provide a trial basis. 1 Materials Agilent 1100 series high performance liquid chromatography. Chuanxiong-based Research from callus culture, subculture, respectively 8th generation, the 9th generation, 10th generation, ferulic acid reference substance (China Phar